Laboratory technicians and the genealogist told police they believed one of the Hartman brothers killed Welch and recommended obtaining DNA samples from them to compare to the DNA sample from the crime scene. Detectives began running surveillance on the Hartman brothers in early June. After Hartman and the co-worker left, the detective got the paper bag — and the paper napkin — from a server cleaning the lobby. He was arrested Wednesday during a traffic stop in Lakewood.
You will compare the DNA found inside of the bones of your skeleton to the two individuals who match the profile you have provided. DNA samples provided by the family of those who have gone missing will each be cut, or digested, with two restriction enzymes in separate reactions and will be compared to DNA isolated from the humerus of the unearthed skeleton.
The DNA extracted from your skeleton has already been digested with these same two enzymes. Analyze DNA from the two individuals who have been linked to your skeleton: Missing Person 1 and Missing Person 2.
Obtain four, clean microcentrifuge tubes. All other reagents should be located at your lab station. Label microcentrifuge tubes for four restriction enzyme digestion reactions.
Make sure to label each tube with the name of your group. Also, make sure to note the contents of each tube in your laboratory journal. You will process four DNA samples: Add DNA and enzymes to tubes according to the following information.
Remember to use a fresh pipette tip for each transfer. You do not want to contaminate your samples. Cap each tube and gently tap the side of each tube to mix. Tap the tube gently on the desk to make sure all of the contents are in the bottom of the tube.
Place your tubes in the bath as directed by your teacher. Depending on time, your teacher may complete this step for you. As your samples digest, continue with Steps 8 Look at Act 1. With your partner, research the science behind restriction enzymes.
Use the websites listed below to complete the questions and activities in Part A of the Student Response Sheet. Review the process of gel electrophoresis by viewing the animations listed below.
Use information from the sites to complete the activity described in Part B of the Student Response Sheet. Remove the comb carefully from the agarose gel and place the gel in the electrophoresis chamber.
Fill the chamber with TAE buffer, making sure the gel is completely submerged.
Obtain your sample tubes Gently flick the side of each tube to mix the contents. Your teacher will direct you to the heat block or water bath. Allow the samples to cool a few minutes before loading them on the gel.
While your samples cool, practice loading samples on the practice gels.
Make sure to use a fresh tip for each sample. Draw a diagram of the gel in your lab notebook. Make sure to clearly indicate which sample is in which well. Be sure to check the orientation of your gel.
The DNA containing wells should be closer to the negative pole and farther away from the positive pole. Check the DNA samples 5 minutes after turning on the power supply.Conclusion Questions 1. Explain the relationship between the following words – cells, genes, chromosomes, tissues, DNA, proteins.
Tissue is made up of cells that have a specific function. Project Mystery Disease Part 2: Genetic Testing- In this project students will learn about Huntington’s disease and investigate how DNA is isolated for genetic testing by collecting cheek cells, isolating DNA, and creating a necklace with their isolated DNA.
1 3 1 case report- dna detectives - Free download as Word Doc .doc /.docx), PDF File .pdf), Text File .txt) or read online for free. Scribd is the world's largest social reading and publishing site. This artifact includes a venn diagram of comparing the two types of diabetes.
It shows how type 1 and type 2 are different and the same. It includes the definition, symptoms, and types of . Research question one: What is DNA? .. Research question two: What is the importance of DNA evidence as an investigative tool?
was found that detectives on the detective courses were given training in the collection and preservation of . Los Angeles Police Department Manual (2nd Quarter ): Volumes 1 through 4.
2. LAPD Detective Operations Manual (December ): Volumes I DNA Filing Procedures (6/22/11); Cellular Telephone Tracking Requests (10/16/12); Cross-reference of DR3 with Employee Travel, – Detective Hours, – FLSA Agreement, and – Off.